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KMID : 0366119930210010013
Korean Journal of Applied Microbiology & Bioengineering
1993 Volume.21 No. 1 p.13 ~ p.17
Enzymatic Reactions in Citric Acid Fermentation of Mandarin Orange Peel by Aspergillus niger
°­½Å±Ç:Kang Shin-Kwon
³ëÁ¾¼ö:Roh Jong-Soo/¼º³«°è:Sung Nack-Kie
Abstract
When mandarin orange peel was used for a substrate of citric aCid fermentation by Aspergillus niger, principal enzyme activities were investigated. Not only the activity of polygalacturonase and pectin esterase being capable of digesting pectin and crude fiber of mandarin orange peel. but also that of carboxymethyl cellulase, xylanase and amylase was high. In carbohydrate metabolism, the activity of enzymes related in HMP pathway was higher than that in EMP pathway at the orange peel medium designed hereby rather than synthetic medium. Productivity of citric acid was significantly increased when the activity of citrate synthetase was high and 5imultaneously those of aconitase and NADP-dependent dehydrogenase were low.
KEYWORD
Orange peel, citric acid fermentation, Aspergillus niger
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